University of Birmingham > Talks@bham > Medical Imaging Research Seminars > DNA Barcodes: Separation and identification of bacteriophage DNA

DNA Barcodes: Separation and identification of bacteriophage DNA

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If you have a question about this talk, please contact Matthaios Doulgerakis.

DNA methyltransferases can be used to label DNA sequence-specifically with, for instance, fluorescent probes. This is a novel technology which allows DNA to be targeted without damage, with high efficiency, and crucially allows sequence context. If fluorophore-labelled DNA is combed onto a surface, single molecules of DNA can be used as a ‘barcode’, and read and analysed much like any other barcode. Currently, the leading approaches for identification of microorganisms are next generation sequencing methods. However, typically short sequence reads are used and can make sequence assembly difficult, particularly in complex and repetitive genomes. The DNA barcode can act as a complementary technique, allowing direct visualisation of the DNA sequence. I will describe a new approach which can rapidly separate and identify mixtures of genomic DNA .

This talk is part of the Medical Imaging Research Seminars series.

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