University of Birmingham > Talks@bham > Medical Imaging Research Seminars > Dulux or Da Vinci? Enhancing resolution with oligonucleotide sequences.

Dulux or Da Vinci? Enhancing resolution with oligonucleotide sequences.

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If you have a question about this talk, please contact Matthaios Doulgerakis.

Numerous techniques exist to break the diffraction limit of light, however most subject samples to high intensity lasers, inducing phototoxicity and eventual sample degradation. DNA -PAINT (Point Acquisition for Imaging Nanoscale Topography) relies on the stochastic annealing of short (12-20 base pairs) oligonucleotides labelled with fluorophores to achieve the conditions necessary for super resolution imaging, circumventing the need for high power laser illumination. This presentation will show recent work in optimising the application of the DNA -PAINT technique to cellular systems, and detail future work in the combination of the sister technique qPAINT with existing cluster analysis.

This talk is part of the Medical Imaging Research Seminars series.

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