University of Birmingham > Talks@bham > School of Chemistry Seminars > PTC Seminar: Microfluidic Technologies for the Bottom-Up Construction of Artificial Cells and Single Cell Analysis

PTC Seminar: Microfluidic Technologies for the Bottom-Up Construction of Artificial Cells and Single Cell Analysis

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  • UserDr Oscar Ces, Department of Chemistry, Imperial College
  • ClockThursday 19 May 2016, 14:00-15:00
  • HouseHaworth 203.

If you have a question about this talk, please contact Dr Dwaipayan Chakrabarti.

PTC Seminar hosted by Dr Melanie Britton

The first part of this talk will outline microfluidic strategies for bottom-up synthetic biology that are being used to construct multi-compartment artificial cells where the contents and connectivity of each compartment can be controlled. These compartments are separated by biological functional membranes that can facilitate transport between the compartments themselves and between the compartments and external environment. These technologies have enabled us to engineer multi-step enzymatic signalling cascades into the cells leading to in-situ chemical synthesis and systems that are capable of sensing and responding to their environment. In addition, we have developed printing strategies for translating these enzymatic pathways into microfluidic flow reactors that have the potential to be scaled-up for industrial usage.

The second half of this talk will present a novel microfluidic platform technology, the microfluidic antibody capture chip (MAC Chip), a generic platform capable of undertaking the analysis of protein copy number from single cells. The approach involves high-speed lysis of single cells in separate analysis chambers through laser induced microcavitation. The protein released from each single cell is then measured by total internal reflection microscopy as it is bound to micro-printed antibody spots within the device. The system can be used to analyse the protein content from single cells or cell ensembles.

This talk is part of the School of Chemistry Seminars series.

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